Berkman twisted his right ankle when he stepped on pitcher Bryan Bullington's foot on first base on Sunday while trying to beat out a double play. Marcus Thames pinch-hit for the designated hitter the next time his spot in the order came up.
Manager Joe Girardi said that Berkman remains day-to-day.
"I don't expect it to be a long time, but I don't know if it's going to be two days or one day or three days," Girardi said. "I doubt I would have him today [as a pinch-hitter]."
With Berkman out of the lineup, Jorge Posada started at DH on Monday night with Francisco Cervelli behind the plate.