Dickerson pulled up with what the team is describing as cramping and spasms in his left hamstring while running the bases on Saturday following his third hit in three at-bats.
The 28-year-old was acquired from the Brewers on Friday for right-hander Sergio Mitre, and he drew the start against the Pirates, playing left field. He will be re-evaluated on Sunday.
"We think it's cramps, but obviously we're not going to know until [Sunday]," Yankees manager Joe Girardi said. "He was actually cramping up this morning a little bit and did not inform anyone."
Dickerson was obtained in part to be an insurance policy for the Yankees, who are not sure if center fielder Curtis Granderson will be healed from a strained right oblique in time for Opening Day.
He doubled in his first at-bat against Pittsburgh's Kevin Correia, then added a pair of singles, driving in a run.
Earlier, Dickerson said he did not know how much of a chance he'd have of going north with the team. He was originally drafted by the Yankees in 2000, but elected to attend the University of Nevada-Reno instead.
"I was drafted out of high school by the Yankees, and to be in this position now is really special," Dickerson said.