Matsui started in left field for New York and injured the hamstring during his lone at-bat of the contest, grounding out to catcher Alberto Castillo in the second inning.
The Yankees said that Matsui will be re-evaluated on Sunday, though he said that the injury does not appear to be severe.
"The pain is not too serious," Matsui said. "It should be fine by then."
Even so, Yankees manager Joe Torre said that in a best-case scenario, the team might be without Matsui's services for three to five days.
With only one reserve outfielder -- Melky Cabrera -- on the 25-man roster, utilityman Miguel Cairo could garner playing time in Matsui's absence.
Cairo replaced Matsui as a pinch-hitter in the fourth inning and took over in left field, going 0-for-2 before Johnny Damon -- who has been tending an injury of his own, a strained right calf, entered the game as a pinch-hitter.
"I will be ready for it," Cairo said. "I've been working out hard. I had a good day today."
Matsui played in just 51 games for New York last season after suffering a broken left wrist in a May 11 contest against the Boston Red Sox, costing him four months and snapping a consecutive games played streak of 518 games to open his Major League career.
Matsui batted .302 with eight home runs and 29 RBIs in 2006. In four games this season, he is 3-for-12 (.250) with three runs batted in.
Bryan Hoch is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.