Rodriguez, who had struck out and hit a run-scoring double in his first two-at bats, reached on a fielder's choice in the sixth inning after Bobby Abreu hit a leadoff single. While Hideki Matsui was batting, Rodriguez appeared to feel some discomfort in his right leg.
After stepping off the base to lead off, Rodriguez called timeout and flexed his right leg a couple of times. One pitch later, he summoned the trainer out of the Yankees' dugout.
Assistant trainer Steve Donohue and manager Joe Girardi jogged toward first base and spoke briefly with Rodriguez before removing him for pinch-runner Morgan Ensberg.
Pete Kerzel is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.