The 39-year-old left-hander is scheduled to throw 90 to 95 pitches in the start against the Portland Sea Dogs, an affiliate of the Red Sox. That contest is set to begin at 6 p.m. ET at Hadlock Field in Portland, Maine.
Pettitte is coming off a start on Wednesday for Trenton in which he fired 59 of 81 pitches for strikes against the Erie Sea Wolves in Trenton, N.J., suffering the loss in a 10-4 defeat. Pettitte allowed four runs (three earned) and seven hits over five-plus innings, striking out three and walking one.
"His plans are, he feels good, he's going to pitch Monday again [for] Trenton and we'll go from there," said Yankees manager Joe Girardi, who spoke to Pettitte on Thursday.
Pettitte is estimated to be on target for a mid-May return to the Major League rotation.