Granderson was forced out of Saturday's game against the Orioles after feeling the hamstring bother him during an at-bat. An MRI exam taken in New York revealed no strain or tear.
"I think he's pain-free, but I still think you have to keep an eye on him," Yankees manager Joe Girardi said. "I'll probably check with him a couple of times during the game to see if he's OK."
Granderson entered play on Tuesday batting .234 with a team-leading 34 home runs and 79 RBIs.