Infielder David Adams was optioned to Triple-A Scranton/Wilkes-Barre after Sunday's 6-5 win over the Rays.
Nix began running on Tuesday, and he played rehab games in Class A on Thursday, Friday and Saturday. He went 1-for-10 in those games with a double and an RBI.
He's batting .236 with two home runs, 20 RBIs and 11 stolen bases with the Yankees this season.
"I never had any kind of setbacks or any kind of days where I didn't feel that good," Nix said. "As soon as I started doing stuff, I felt great."
Josh Vitale is an associate reporter for MLB.com. David Wilson is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.