Chamberlain barked at first-base umpire Joe West in the 10th inning of New York's 9-8 loss to Boston, believing West missed the call as Shane Victorino appeared to offer at an 85-mph slider that would have gone for strike three and the second out.
Instead, given new life, Victorino punched a 96-mph fastball for a single into right field that scored Jacoby Ellsbury with the go-ahead run.
"I'd seen Shane move up in the box, so I kind of wanted to get a ball up," Chamberlain said. "I saw him swung through on [David Robertson] earlier in the day. I was trying to do that."
Manager Joe Girardi said that he also thought West missed the swing by Victorino. Even Red Sox manager John Farrell called it "a borderline call" for West to make, admitting, "We may have caught a break there."
"It's frustrating, because I thought he went, but you have to be able to overcome things," Girardi said.
Chamberlain, the seventh Yankees pitcher of the night, was in the game because three of Boston's next four hitters entering the 10th inning were right-handed.
Girardi said that he thought it was "pretty tough" to put Phil Hughes in that situation and that Shawn Kelley is unavailable due to a triceps injury, so Chamberlain got the nod.
"Obviously you execute the way you wanted and it doesn't go your way. It's extremely frustrating," Chamberlain said.
Bryan Hoch is a reporter for MLB.com. Follow him on Twitter @bryanhoch and read his MLBlog, Bombers Beat. Josh Vitale is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.